ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2016, Vol. 47 ›› Issue (3): 477-483.doi: 10.11843/j.issn.0366-6964.2016.03.008

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Expression Stability Analysis of Reference Genes in Different Development Periods and Tissues in Oryctolagus Cuniculus

CHEN Rui,YANG Xiao-nong*,ZENG Wan-qiu,WEN Juan   

  1. (College of Life Science and Technology,Southwest University for Nationalities,Chengdu 610041,China)
  • Received:2015-03-04 Online:2016-03-23 Published:2016-03-23

Abstract:

The purpose of this study was to screen the most stable reference genes in different development periods and tissues in New Zealand White rabbit.Quantitative real-time Polymerase Chain Reaction(RT-qPCR),a latest online reference genes stability assessment tool RefFinder and Minimum Information for Publication of Quantitative Real-time PCR Experiments(MIQE) guideline were used to compare the expression levels of 6 commonly used reference genes(GAPDHHPRT1,18S rRNAB2MSdhaβ-actin) mRNA in 3 tissues(heart,liver,kidney) of rabbit at embryo(E28.5),juvenile(10 d) and adult stages(A).The result showed that the most stable reference genes in liver and kidney of New Zealand White rabbit in 3 development periods were β-actinHPRT1 and GAPDH,successively.But for heart,they were β-actinHPRT1, Sdha.The most stable reference genes in embryo period of New Zealand White rabbit in 3 tissues were GAPDHβ-actin and HPRT1,successively.For juvenile and adult stages,they were HPRT1,GAPDH and β-actin. To all 3 periods and all 3 tissues of New Zealand White rabbit,the most stable reference genes were successively β-actinHPRT1 and GAPDH.This study confirmed that the most stable reference genes were different in different periods and tissues of New Zealand White rabbit.To ensure minimal variation between individuals in an experimental group and the accurate statistical analysis of small fold changes,we recommend normalizing RT-qPCR data to the geometric mean of at least 3 validated reference genes as above when related New Zealand White rabbit trials were performed.

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